Fig. 6

Detection of BYDV GAV in field samples by ACP-ELISA, dot -ELISA, and RT-PCR. a, Detection of BYDV GAV in field wheat samples through ACP-ELISA. Twenty two field wheat samples were loaded in wells on an ELISA plate. BYDV GAV-infected (CK+) and healthy wheat plants (CK-) were used as the positive and negative controls. b, Detection of BYDV GAV in wheat plant samples through dot-ELISA. Row A 1–8 were 1–8 samples shown in the panel A. B 1–8 were 9–16 samples shown in the panel A and C 1–6 were 17–22 samples shown in the panel A. Row C 7 and 8 were CK+ and CK- shown in the panel A. Dark purple color indicated a positive reaction. c, Detection of BYDV GAV in wheat samples through RT-PCR. Samples used in this assay were the same samples shown in the panel (a) and (b). BYDV GAV CP specific forward and reverse primers were used in this assay. Lane M, 1Kb DNA marker