Fig. 5

Specificity and sensitivity of the dot-ELISA. a, Crude extracts were prepared from the BYDV GAV-, BYDV GPV-, BYDV PAV-, WYMV-, CWMV-, WDV- or BaYMV-infected wheat plants (GAV, GPV, PAV, WYMV, CWMV, WDV and BaYMV) and blotted onto the membrane. Each dot contained 2 μl extract and each sample has two dots (up and lower). Crude extract from healthy wheat plants (CK-) was used as a negative control. b, Aphids fed on the BYDV GAV-, BYDV GPV- or BYDV PAV-infected wheat plants (GAV, GPV and PAV) were used for this assay. Each dot contained 2 μl extract and each sample has two dots (up and lower). Extract from aphids fed on the healthy wheat plant (CK-) was used as a negative control. c, BYDV GAV-infected (CK+) and healthy wheat (CK-) plants were used in this assay. Crude extracts from the GAV or CK- plants were two-fold diluted from 1:40 to 1:10,240 (w/v) in 0.01 mM PBS prior to the assay. The membrane was probed with the monoclonal antibody 18A1 followed by the goat anti-mouse IgG/AP or HRP conjugate