Fig. 6From: Expression of chicken interleukin-2 by a highly virulent strain of Newcastle disease virus leads to decreased systemic viral load but does not significantly affect mortality in chickensPhotomicrographs illustrating immunohistochemistry (IHC) for NDV NP (first column of panels) and IL-2 (second column of panels) on sections of eyelids. Tissues were harvested from 4-week-old White Leghorn chickens infected with rZJ1-IL2 (a-d) and rZJ1-GFP (e, f) at day 5 pi. Alkaline phosphatase method and hematoxylin counterstain. At low magnification, in the eyelids of rZJ1-IL2-infected birds, the same areas that are immunolabeled for NDV NP are also positive for chicken IL-2 (dotted circles). At higher magnification, scattered cells in consecutive sections are immunolabeled for both NDV NP and IL-2 (arrows). No signal for IL-2 is observed in sections of eyelids from rZJ1-GFP-infected animals (f)Back to article page