Fig. 1From: Application of recombinant severe fever with thrombocytopenia syndrome virus nucleocapsid protein for the detection of SFTSV-specific human IgG and IgM antibodies by indirect ELISAExpression and purification of recombinant SFTSV-N protein. a Schematic figure of the recombinant expression plasmid with the SFTSV-N insert. b SDS-PAGE analysis of recombinant SFTSV-N protein. The proteins from the supernatant and pellet of the recombinant expressing E. coli cell lysate and the purified recombinant protein were analyzed in a 5–20 % gradient SDS-PAGE gel which was stained with Coomassie brilliant blue. Lane 1: protein marker (Precision plus protein standards, Bio-Rad); Lane 2: supernatant of sonicated E. coli cell lysate after centrifugation; Lane 3: pellet of sonicated E.coli cell lysate; Lane 4: purified recombinant proteinBack to article page