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Fig. 1 | Virology Journal

Fig. 1

From: Application of recombinant severe fever with thrombocytopenia syndrome virus nucleocapsid protein for the detection of SFTSV-specific human IgG and IgM antibodies by indirect ELISA

Fig. 1

Expression and purification of recombinant SFTSV-N protein. a Schematic figure of the recombinant expression plasmid with the SFTSV-N insert. b SDS-PAGE analysis of recombinant SFTSV-N protein. The proteins from the supernatant and pellet of the recombinant expressing E. coli cell lysate and the purified recombinant protein were analyzed in a 5–20 % gradient SDS-PAGE gel which was stained with Coomassie brilliant blue. Lane 1: protein marker (Precision plus protein standards, Bio-Rad); Lane 2: supernatant of sonicated E. coli cell lysate after centrifugation; Lane 3: pellet of sonicated E.coli cell lysate; Lane 4: purified recombinant protein

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