Fig. 3

Bulge and loop mutations in 5’UTR of HIV-1 genomic RNA resulted in reduced association of eEF1A and RT in virus infected cells. a TZM-bl cells were infected with WT, Loop-M, Bulge-M virus as indicated equal to 100 ng CAp24. Association of eEF1A with HIV-1 RTC in virus infected TZM-bl cells was determined by proximity ligation assay using anti-eEF1A and anti-RT antibodies at 2 h of post-infection. A red foci represents a positive signal that was visualized using a DeltaVision Core imaging system and analysis was performed from more than 200 cells. b The levels of genomic RNA at 2 h of post-infection were determined by RT-PCR and possible DNA contamination was examined by PCR without RT