Fig. 3
From: DAXX modulates human papillomavirus early gene expression and genome replication in U2OS cells
The localization of HPV replication foci in U2OS cells in relation to the PML protein. Immunofluorescene analysis of U2OS cells transfected with HPV11 and HPV18E8- genomes. a and b U2OS cells were transfected with wt HPV11 minicircle genomes and grown on coverslips. Six days after transfection cells were fixed and immunostained with antibodies for HPV11 E2 (green) and PML (red) proteins. Cell nuclei were detected by DAPI. Analysis was carried out at least three times starting from cell transfections. a and b represent cells with different number and size of HPV11 E2 foci. c, d, e and f U2OS cells were transfected with HPV18E8- minicircle genomes and grown on coverslips. Six days after transfection cells were fixed and immunostained with antibodies for HPV18 E1 (green) and PML (red) proteins. Cell nuclei were detected by DAPI. Analysis was carried out at least three times starting from cell transfections. c, d, e and f represent cells with different number and size of HPV18 E1 foci. On the right panels, the intensity profiles are shown