Fig. 6

Agarose gel electrophoresis showing 17 positive field specimens detected by the developed multiplex PCR. A volume of 3 μl DNA was as template in a total reaction volume of 50 μl. Lane M; 100 bp marker, NTC; non-template control (all PCR components except template DNA), Lanes 1–5; specimens positive for CPPV, Lane 6; Field specimen with CPPV and CdPV co-infection, Lanes 7–11; specimens positive for CdPV, Lanes 12–17; specimens positive for CMLV. PCR products were resolved by electrophoresis in 1.5 % agarose in tris-acetate EDTA (TAE) buffer (40 mM Tris–acetate pH 8.0,1 mM EDTA) and the gel was stained with ethidium bromide and photographed