Fig. 2
Agarose gel electrophoresis showing optimization of annealing temperature for the three viruses in the multiplex assay. A volume of 1 ul plasmid DNA containing 106 plasmid copy numbers of CPPV, CdPV and CMLV was added to the PCR and the assay was run in a gradient thermal cycler (BIO-RAD 100) using different annealing temperatures indicated above the figure. PCR pruducts were 569, 478 and 226 bp for CPPV, CdPV and CMLV, respectively. Lane M; 100 bp marker. PCR products were resolved by electrophoresis in 1.5 % agarose in tris-acetate EDTA (TAE) buffer (40 mM Tris–acetate pH 8.0,1 mM EDTA) and the gel was stained with ethidium bromide and photographed CPPV; Camel parapox virus, CdPV; Camelus dromedary Papilloma Virus, CMLV; Camelpox virus