Table 3 Primers used in the study
From: Efficient generation of influenza virus with a mouse RNA polymerase I-driven all-in-one plasmid
Target/template | Primer | Sequence (5′ → 3′)a |
|---|---|---|
MTI-EGFP/pYA4392 | P1 | ttgtcgcccggagtactggtcg |
P2 | attggacctggagataggtagtagaaacaaggtag | |
MPI/mouse genomic DNA | P3 | ctaccttgtttctactacctatctccaggtccaat |
P4 | tacgtctgaggccgagggaaagc | |
SV40 pA/pcDNA3.1(−) | P5 | tacagacatgataagatacat |
P6 | cctcggcctcagacgtaaacttgtttattgcagc | |
CMV/pcDNA3.1(−) | P7 | caacttcggaggtcgaccagtactccgggcgacaagagctctgcttatatag |
P8 | ttaagatctgtacatcaatgggcgtgg | |
MPI/pYA4924 | P9 | gctgcaataaacaagtttacgtctgaggccgagg |
P10 | tggtcgacctccgaagttgggggggtgagacggatccgtctctacctatctccaggtcc | |
GFP cassette/pYA4332 | BglII-lpp | taaagatctttgttgtgtgaattaat |
BglII-5ST1T2 | ttaagatcttccattattgaagcatt | |
PB1/pYA4384 | AarI-1 PB1 | taacacctgcagtcaggtagtagaaacaaggcatt |
AarI-2 PB1 | ttacacctgcgactggggagcgaaagcaggcaaac | |
PB2/pYA4383 | AarI-1 PB2 | taacacctgcagtcaggtagtagaaacaaggtcgt |
AarI-2 PB2 | ttacacctgcgactggggagcgaaagcaggtcaat | |
PA/pYA4385 | BsmBI-1PA | taacgtctctaggtagtagaaacaaggtact |
BsmBI-2PA | ttacgtctctggggagcgaaagcaggtactg | |
NP/pYA4386 | BsmBI-1NP | taacgtctctaggtagtagaaacaagggtat |
BsmBI-2NP | ttacgtctctggggagcaaaagcagggtaga | |
HA/pYA4388 | BsmBI-1HA | taacgtctctaggtagtagaaacaagggtg |
BsmBI-2HA | ttacgtctctggggagcaaaagcaggggaa | |
NA/pYA4389 | AarI-1NA | taacacctgcagtcaggtagtagaaacaaggagtt |
AarI-2NA | ttacacctgcgactggggagcgaaagcaggagttt | |
M/pYA4390 | BsmBI-1 M | taacgtctctaggtagtagaaacaaggtagt |
BsmBI-2 M | ttacgtctctggggagcaaaagcaggtagat | |
NS/pYA4391 | BsmBI-1NS | taacgtctctaggtagtagaaacaagggtgt |
BsmBI-2NS | ttacgtctctggggagcaaaagcagggtgac | |
NP cassette /pYA4968 | PmlI-NP | cacgtgtacatcaatgggcgtggatagcg |
SmaI-NgoMIV-NP | cccgggatagccggcagacatgataagatacat | |
PA cassette/pYA4967 | NgoMIV-PA | taagccggcgtacatcaatgggcgtggat |
GGG-AsiSI-PA | gggatagcgatcgcagacatgataagatacat | |
PB1 cassette/pYA4965 | AsiSI-PB1 | gcgatcgcgtacatcaatgggcgtggat |
SmaI-NotI-PB1 | cccgggatagcggccgcagacatgataagatacat | |
PB2 cassette/pYA4966 | NotI-PB2 | taagcggccgcgtacatcaatgggcgtggat |
GGG-BssHII-PB2 | gggatagcgcgcagacatgataagatacat | |
NS cassette/pYA4972 | BssHII-NS | gcgcgcgtacatcaatgggcgtggatag |
SmaI-KpnI-NS | cccgggataggtaccagacatgataagatacat | |
M cassette/pYA4971 | KpnI-M | taaggtaccgtacatcaatgggcgtggat |
GGG-PacI-M | gggatattaattaacagacatgataagatacat | |
NA cassette/pYA4970 | PacI-NA | ttaattaagtacatcaatgggcgtggatag |
SmaI-SbfI-NA | cccgggatacctgcaggcagacatgataagatacat | |
HA cassette/pYA4969 | SbfI-HA | taacctgcagggtacatcaatgggcgtggat |
GGG-HA | gggcagacatgataagatacattgatg | |
GFP cassette/pYA4964b | GGG-5ST1T2 | gggtccattattgaagcatttatcaggg |
SbfI-lpp | taacctgcaggttgttgtgtgaattaatttgt | |
KpnI-lpp | taaggtaccttgttgtgtgaattaatttgt | |
NotI-lpp | taagcggccgcttgttgtgtgaattaatttgt | |
NgoMIV-lpp | taagccggcttgttgtgtgaattaatttgt | |
PacI-lpp | taattaattaattgttgtgtgaattaatttgt | |
BssHII-lpp | taagcgcgcttgttgtgtgaattaatttgt | |
AsiSI-lpp | taagcgatcgcttgttgtgtgaattaatttgt |