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Table 2 Maximum intra and inter assay variations in different concentrations of plasmid DNA and viral RNA

From: Validation and utilization of an internally controlled multiplex Real-time RT-PCR assay for simultaneous detection of enteroviruses and enterovirus A71 associated with hand foot and mouth disease

Concentration

Plasmid DNA (copies)

Viral RNA (TCID50)

4 × 106

4 × 103

4 × 101

9.6 × 105

9.6 × 103

9.6 × 101

Maximum EV assay co-variation

Intra-assay

0.53

0.93

1.37

1.49

0.84

0.77

Inter-assay

3.13

1.36

2.18

4.41

3.01

2.54

Maximum EV-A71 assay co-variation

Intra-assay

0.52

1.00

2.18

1.34

0.73

0.73

Inter-assay

3.19

1.37

1.85

2.66

1.56

1.53

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Virology Journal

ISSN: 1743-422X