Table 1 Primers and RT-PCR (generic/nested) conditions for arboviral detection in target groups

Flavivirus

Flavi1+ GAYYTIGGITGYGGIIGIGGIRGITGG

1 cycle – 45 min/38°C

[13]

Flavi1− TCCCAICCIGCIRTRTCRTCIGC

40 cycles: 30 sec/94°C, 1 min/47°C, 75 sec/68°C

1 cycle – 5 min/68°C

Flavi2+ YGYRTIYAYAWCAYSATGGG

1 cycle – 2 min/94°C

Flavi2− CCARTGITCYKYRTTIAIRAAICC

40 cycles: 30 sec/94°C, 1 min/47°C, 15 sec/72°C

1 cycle – 5 min/72°C