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Table 1 Primers and RT-PCR (generic/nested) conditions for arboviral detection in target groups

From: Evolutionary relationships of West Nile virus detected in mosquitoes from a migratory bird zone of Colombian Caribbean

Arboviruses group Primers Conditions for RT-PCR Reference
Flavivirus Flavi1+ GAYYTIGGITGYGGIIGIGGIRGITGG 1 cycle – 45 min/38°C [13]
Flavi1− TCCCAICCIGCIRTRTCRTCIGC 40 cycles: 30 sec/94°C, 1 min/47°C, 75 sec/68°C
1 cycle – 5 min/68°C
Flavi2+ YGYRTIYAYAWCAYSATGGG 1 cycle – 2 min/94°C
Flavi2− CCARTGITCYKYRTTIAIRAAICC 40 cycles: 30 sec/94°C, 1 min/47°C, 15 sec/72°C
1 cycle – 5 min/72°C