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Figure 2 | Virology Journal

Figure 2

From: Residues 41V and/or 210D in the NP protein enhance polymerase activities and potential replication of novel influenza (H7N9) viruses at low temperature

Figure 2

Viral RNA polymerase activities of NP-41I/41V and/or NP-210E/210D in 293T cells in the backbone of A/Anhui/1/2013 (H7N9). Luciferase-based minigenome reporter assays were used to measure polymerase activity in 293T cells at 33°C (A) or 37°C (B). Cells were co-transfected with Gluc reporter plasmid and expression plasmids PB2, PB1, and PA of A/Anhui/1/2013 (H7N9), plus different NPs to generate different viral RNPs. After culturing at 33°C or 37°C for 24 h, Gaussia luciferase production was measured and normalized to AH1 activity. Results are presented as mean ± SEM and are representative of three independent experiments.

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Virology Journal

ISSN: 1743-422X