Enhanced humoral response in pregnant mice immunized with liposome encapsulated recombinant neutralizing epitope protein of Hepatitis- E virus

Table 3 Cytokine levels in the sera / supernatants of stimulated-splenocytes and expression of corresponding genes in the spleens of non-pregnant and pregnant immunized mice at different time points

Category and time after the dose	Serum cytokines (pg/ml)			Gene expression spleen (fold change)			Splenocytes (rNEp-stimulated , pg/ml)
Category and time after the dose	IL-2	IL-4	IFN-γ	IL-2	IL-4	IFN-γ	IL-2	IL-4	IFN-γ
NPR 24 hrs*	UD	UD	UD	7.6D	2.7D	2.8U	5	5	4.7
NPR 2 weeks	UD	UD	UD	2.5D	5.7D	3.1D	5	10	30
PI 24 hrs**	19.4	UD	56.6	0.7	3.7D	0.9	5	4.5	4.8
PI 2 weeks	2.7	UD	6.2	3.2D	0.9	2.9D	4.5	20	10
PNI day 8***	22.2	UD	UD	2.6D	2.2D	3.2D	4.8	4.5	4.2
PNI day 18	4.7	UD	UD	4.3D	4.2D	3D	5	4.5	4.8

*indicates non-pregnant immunized mice, **indicates pregnant immunized mice, ***indicates pregnant unimmunized mice. UD = undetectable, D = downregulation, U = upregulation.

ISSN: 1743-422X