Enhanced humoral response in pregnant mice immunized with liposome encapsulated recombinant neutralizing epitope protein of Hepatitis- E virus

Table 1 Estimation of serum Th1/Th2 cytokine levels

Groups	Time point	Cytokine levels pg/ml (Std.error)
		IL-2	IFN-γ	IL-12p40	IL-12 p70	IL-6	IL-10
Naiive		0.2(0)	0.2(0)	0.2(0)	0.2(0)	0.2(0)	12.0 (2.3)
NPR*	24 hrs	0.2(0)	0.2(0)	0.2(0)	0.2(0)	20.9 (1.92)	4.1 (1.1)
NPR	2 wks	0.2(0)	0.2(0)	0.2(0)	0.2(0)	8.3 (0.2)	10.2 ( 0.1)
PI**	24 hrs	19.4 (1.1)	56.6 (5.8)	0.2(0)	103 (7.2)	45.9 (1.4)	11.3 (1.5)
PI	2wks	2.7 (0.2)	6.2 (1.1)	28.9 (2.2)	36.4 (1.4)	25.5 (0.5)	23 (2.5)
PNI***	Day8	22.2 (0.2)	0.2(0)	0.2(0)	30.2 (2.4)	12.9 (0.7)	15.4 (0.4)
PNI	Day18	4.7 (0.2)	0.2(0)	19.6 (0.4)	40.8 (3.1)	0.2(0)	25.6 (2.5)
p-values
naiive vs NPR 24 hrs		N.S	N.S	N.S	N.S	<0.001	N.S
naiive vs NPR 2wks		N.S	N.S	N.S	N.S	<0.001	N.S
NPR 24 hrs vs NPR 2wks		N.S	N.S	N.S	N.S	<0.05	<0.05
PI 24 hrs vs PI 2wks		<0.001	<0.001	<0.001	<0.05	N.S	<0.05
PNI day8 vs PNI day18		<0.001	N.S	<0.05	N.S	<0.05	<0.05
PI 24 hrs vs PNI day8		N.S	<0.001	N.S	<0.001	<0.05	N.S
PI 2wks vs PNI day18		N.S	<0.05	N.S	N.S	<0.001	N.S
NPR 24 hrs vs PI 24 hrs		<0.001	N.S	<0.001	<0.001	<0.05	<0.001
NPR 2wks vs PI 2wks		<0.001	<0.001	<0.001	<0.001	<0.001	<0.05

*indicates non-pregnant immunized group, **PI = pregnant immunized group, ***PNI = pregnant non-immunized mice group.
N.S. = not significant, p > 0.05. (ANOVA test).

ISSN: 1743-422X