Table 2 Sequences of the primers and probe used in this study
Application | Primer name | Length(bp) | Primer/probe sequence(5′ to 3′) |
|---|---|---|---|
Set 1 primers for RT-LAMP | M1-F3 | 20 | GGACACATTCTTGGTGGTCT |
M1-B3 | 20 | CCAACACGTCCGTAGACAAT | |
M1-FIP | 42 | TGGTGCTCCAAGCACTGGAATGACGCGCTTCTCACTACTTCT | |
M1-BIP | 40 | AAGGTTGCTACTGGCGTACAGGTTGTAGTGGCCTTGGCGA | |
Set 2 primers for RT-LAMP | M2-F3 | 18 | ACAGACGCGCTTCTCACT |
M2-B3 | 20 | CCAACACGTCCGTAGACAAT | |
M2-FIP | 39 | AGCGTTACACCAGTTGGTGCTCTTCTGTGATGGGCCGAC | |
M2-BIP | 40 | AAGGTTGCTACTGGCGTACAGGTTGTAGTGGCCTTGGCGA | |
Set 3 primers for RT-LAMP | M3-F3 | 19 | GCGCAGGACACATTCTTGG |
M3-B3 | 19 | TTGGCGACTGTGACGAAAT | |
M3-FIP | 40 | GGAATGCAGACCTGTCGGCCTCAATCCTGAAACAGACGCG | |
M3-BIP | 41 | TGGAGCACCAACTGGTGTAACGGTACGCCAGTAGCAACCTT | |
Set 4 primers for RT-LAMP | M4-F3 | 18 | CCGACAGGTCTGCATTCC |
M4-B3 | 20 | CCAGTGCCAGATGAAGCATT | |
M4-FIP | 42 | CCTGTACGCCAGTAGCAACCTTGAGCACCAACTGGTGTAACG | |
M4-BIP | 41 | ATTTCGTCACAGTCGCCAAGGCGACTGAACGACCAACACGT | |
Set 5 primers for RT-LAMP | M5-F3 | 20 | AGCTTTCAGGTCAATTGGGT |
M5-B3 | 20 | GGAGTGTTAGCGTTACACCA | |
M5-FIP | 42 | TGCGCCACAACCGAATGCTATTCAGCATCCTTATGGCTTGCA | |
M5-BIP | 41 | TCAATCCTGAAACAGACGCGCTTGCTCCAAGCACTGGAATG | |
RT-PCR | P1 | 21 | TTCCCAGCGTAGTTGAGATTG |
P2 | 21 | CGAAGTGGCTCTGGATTTGTT | |
real-time RT-PCR | PED-NF | 24 | CGCAAAGACTGAACCCACTAATTT |
PED-NR | 24 | TTGCCTCTGTTGTTACTTGGAGAT | |
PED-Cy5 | 24 | Cy5-TGTTGCCATTGCCACGACTCCTGC-BHQ3 |