Development and application of SYBR Green I real-time PCR assay for the separate detection of subgroup J Avian leukosis virus and multiplex detection of avian leukosis virus subgroups A and B

Table 5 Detection of ALV from clinical plasma samples

Method	Real-time PCR		Routine PCR			Virus isolation ( *p27* detection)
Method	J-F/J-R	AB-F/AB-R	H5/H7	H5/CAPA	BD-F/BD-R	Virus isolation ( *p27* detection)
Positivity rate	77.5% (31/40)	5% (2/40)	70% (28/40)	5% (2/40)	0% (0/40)	62.5% (25/40)
Total Positivity rate	80% (32/40)		72.5% (29/40)			62.5% (25/40)

ISSN: 1743-422X