Figure 4From: Development and application of SYBR Green I real-time PCR assay for the separate detection of subgroup J Avian leukosis virus and multiplex detection of avian leukosis virus subgroups A and B The minimum virus detection limits of ELISA, routine PCR and real-time PCR. The 692 bp amplicons to GD13-1 strain was detected by routine PCR (data not shown), and the S/P values and virus copies were detected by ELISA and real-time PCR, respectively.Back to article page