Whole genome sequence comparison of ten diagnostic brucellaphages propagated on two Brucella abortus hosts

Table 3 Annotated list of open reading frames showing major variations in synonomous and non-synonomous polymorphisms

Annotation	ORF#	Start	End	Length	Direction	Coding Δ	# Loci ^b	Passage ^c
Putative HNH endonuclease	8	3,170	3,856	687	forward	S	1	N
Putative portal protein	12	6,370	8,694	2,325	forward	N	2	Y
Hypothetical protein	13	8,694	8,912	219	forward	S	1	Y
Structural protein	16	9,822	11,003	1,182	forward	N	1	N
Major head protein	17	11,013	12,023	1,011	forward	N	1	Y
Structural protein	20	13,164	13,823	660	forward	N	1	Y
Structural protein (neck motif)	21	13,827	16,211	2,385	forward	N	5	Y/N
Structural protein (peptidase_S74)^a	23	16,660	17,646	987	forward	N	2	Y/N
Putative peptidoglycan hydrolase	25	18,062	20,251	2189	forward	N	1	Y
Structural protein	26	20,251	21,753	1,503	forward	N	1	Y
Putative tail collar protein	27	21,755	22,612	858	forward	S-N	7	Y/N
Hypothetical protein	39	28,262	28,382	121	reverse	N	1	Y
Hypothetical protein	42	28,727	29,683	957	reverse	S-N	2	Y

^aOrtholog encoded by Brucella 83/13.
^bIndicates number of variable locations oberved within individual ORFs.
^cIndicates loci showing polymorphism among passaged strains (Y) versus variants present only within the TbM and Pr reference strains (N).

ISSN: 1743-422X