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Figure 1 | Virology Journal

Figure 1

From: A general method to eliminate laboratory induced recombinants during massive, parallel sequencing of cDNA library

Figure 1

Experimental method for assessing transfection as a source of artificial chimeras. Virus was produced by transfection of HEK293T cells, harvested and quantitated using an ELISA assay to detect HIV-1 p24CA protein. In these studies RNA was extracted from either a mix of equal amounts of homozygous WT and homozygous MK virus (top) or from an equivalent amount of heterozygous virus (bottom).

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