Figure 1

Experimental method for assessing transfection as a source of artificial chimeras. Virus was produced by transfection of HEK293T cells, harvested and quantitated using an ELISA assay to detect HIV-1 p24^CA protein. In these studies RNA was extracted from either a mix of equal amounts of homozygous WT and homozygous MK virus (top) or from an equivalent amount of heterozygous virus (bottom).