Figure 3
Late IFN/ISG and pro-inflammatory cytokine response to infection with WNV NY99 and WNV NSW2011 in human MoDCs. MoDCs from three separate donors were infected with WNVNY99 or WNVNSW2011 at MOI = 1 and total cellular RNA was isolated at 48 and 72 hours post-infection. IFNβ (a), OAS1 (b), MxA (c), TNFα (d) and IL-6 (e) mRNA levels as determined by qRT-PCR were normalised to a combination of three endogenous controls (TBP, GAPDH and PPIA), and expressed as fold change from uninfected samples. The results show mean values from three different donors (donors 1 to 3) and error bars represent standard error of the mean. A Student’s t-test was performed to determine statistical significance at each time point (*: P ≤ 0.05).