Figure 5

Time-course of bp53 expression in gill and hemolymph after WSSV challenge. A relative quantitative real-time PCR assay was applied to study bp53 differential expression profiles in L. vannamei hemolymph (A) and gill (B) in response to WSSV infection within 24 h. Gene expression quantification was determined using the 2^−ΔΔCt method. Actin was used as an internal control. Error bars indicate standard deviations (n = 3). Significant differences between the expression level in each time point post injection and the original level were indicated with an asterisk (p < 0.05).