Hepatosplenomegaly in rabbit 419. Splenomegaly was noted at autopsy in 3 of the 4 rabbits that survived the 15 day cyclosporin A (CsA) treatment. In rabbit 419, both the spleen and liver were enlarged (A). EBV BamH1 W PCR on DNA extracted from rabbit spleens indicated that all were EBV positive (B). Amplification signals were stronger in the spleens of the 4 animals that survived the 15 day immunosuppressive treatment compared to the 2 animals (Rbt 417 and Rbt 418) that died on day 2 after the start of the CsA treatment. Similar results were observed using two different sets of EBV BamH1 W primers (amplicon size of 552 bp and 152 bp). Quantitative PCR analysis (C) confirmed the standard end-point PCR findings observed in Figure 4B.