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Table 3 Pathogens used and Ct values of the duplex real-time PCR assay’s specificity

From: A duplex real-time PCR assay for the detection and quantification of avian reovirus and Mycoplasma synoviae

Pathogen

Number of samples

Ct values of duplex real-time PCR assay

MS

ARV

NDV

1

Negative

Negative

H9 subtype of AIV

1

Negative

Negative

AIBV

1

Negative

Negative

IBDV

1

Negative

Negative

MG

1

Negative

Negative

MM

1

Negative

Negative

MI

1

Negative

Negative

MDV

1

Negative

Negative

pMD18-T-ARV

1

Negative

15.58

pMD18-T-MS

1

15.31

Negative

REV

1

Negative

Negative

ALV

1

Negative

Negative