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Table 1 Specific primers used to clone ARV and MS specific genes

From: A duplex real-time PCR assay for the detection and quantification of avian reovirus and Mycoplasma synoviae

Primer name Primer sequence Amplicon length Target gene
ARV C703F 5’-TGTGGATCCATGGCGGGTCTCAAT-3’ 981 bp σC gene
ARV C703R 5’-CCGGAATTCTAAGGTGTCGATGCC-3’
MS vlhA F2 5’-CTGTTATAGCAATTTCATGTGGTG-3’ 283 bp phase-variable surface lipoprotein hemagglutinin (vlhA)
MS vlhA R2 5’-TGTTGTAGTTGCTTCAACTTGTCT-3’