Table 1 Specific primers used to clone ARV and MS specific genes
Primer name | Primer sequence | Amplicon length | Target gene |
|---|---|---|---|
ARV C703F | 5’-TGTGGATCCATGGCGGGTCTCAAT-3’ | 981 bp | σC gene |
ARV C703R | 5’-CCGGAATTCTAAGGTGTCGATGCC-3’ | ||
MS vlhA F2 | 5’-CTGTTATAGCAATTTCATGTGGTG-3’ | 283 bp | phase-variable surface lipoprotein hemagglutinin (vlhA) |
MS vlhA R2 | 5’-TGTTGTAGTTGCTTCAACTTGTCT-3’ |