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Figure 1 | Virology Journal

Figure 1

From: A small molecule inhibitor of dengue virus type 2 protease inhibits the replication of all four dengue virus serotypes in cell culture

Figure 1

DENV-2 NS2b-NS3Pro enzyme assay, optimization and validation. (A) Kinetics of NS2b-NS3Pro action as a function of substrate concentration (at 5nM enzyme). (B) Rate of enzyme catalysis as a function of enzyme concentration (at 10 μM substrate). (C) Activity of the cloned NS2b-NS3Pro as function of aprotinin concentration (5nM enzyme, 10 μM substrate, 20 min incubation). Activity in the absence of aprotinin was taken as 100% (RFU = relative fluorescence units).

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Virology Journal

ISSN: 1743-422X