Table 3 qPCR confirmation of altered gene expression detected by microarray
Gene symbol | KEGG pathways the validated genes involved | Group comparison | Fold change by microarray | Fold change by qPCR |
|---|---|---|---|---|
IL6 | Cytokine- cytokine receptor interaction; HIF-1 signaling; FOXO signaling; PI3K-Akt signaling; Toll-like receptor signaling | HIVvsHH | 5.1 | 4.8 |
IL6 | HIVvsCTR | 4.6 | 3.5 | |
CXCR4 | Cytokine-cytokine receptor interaction; Chemokine signaling; Endocytosis; Axon guidance; Leukocyte transendothelial migration | HCVvsCTR | −1.5 | −2.5 |
TAOK1 | MAPK signaling pathway | HCVvsHH | −2.0 | −3.7 |
TAOK1 | HIVvsHH | −2.2 | −2.3 | |
EGR2 | Hepatitis B; HTLV-I infection; Viral carcinogenesis | HCVvsHH | 8.8 | 10.2 |
EGR2 | HIVvsHH | 8.8 | 9.3 | |
PPP3CB | MAPK signaling; WNT signaling; Apoptosis; Calcium signaling | HHvsCTR | 1.8 | 1.4 |
WNT7A | WNT signaling; Hedgehog signaling; Hippo signaling; HTLV-I infection | HCVvsHH | 2.1 | 2.6 |
DUSP2 | MAPK signaling pathway | HCVvsHH | 2.6 | 2.8 |
DUSP2 | HIVvsHH | 2.1 | 2.7 | |
DUSP2 | HCVvsCTR | 2.5 | 3.2 | |
MCM2 | DNA replication; Cell cycle | HCVvsHH | −1.5 | −2.9 |
MCM2 | HIVvsHCV | 1.5 | 1.9 | |
GZMB | NK cell mediated cytotoxicity; Regulation of actin cytoskeleton; Transcriptional misregulation in cancer; Autoimmune thyroid disease | HCVvsHH | −2.4 | −3.1 |
GZMB | HIVvsHH | −1.5 | −2.0 | |
GZMB | HIVvsHCV | 1.6 | 1.6 | |
KLRD1 | NK cell mediated cytotoxicity pathway; Antigen processing and presentation; Graft-versus-host disease | HCVvsHH | −2.2 | −2.9 |
KLRD1 | HIVvsHH | −1.3 | −1.5 | |
KLRD1 | HIVvsHCV | 1.6 | 2.0 | |
WEE1 | Cell cycle | HIVvsHCV | 1.3 | 1.9 |
SESN1 | p53 signaling | HIVvsHH | 1.6 | 2.1 |