Table 3 Strains and plasmids
Yeast strain | Genotype | Reporter genes |
|---|---|---|
Y2HGold | MATa, trp1-901, leu2-3, 112, ura3-52, his3-200, gal4Δ, gal80Δ, LYS2::GAL1UAS–Gal1TATA–His3, GAL2UAS–Gal2TATA–Ade2 URA3::MEL1UAS–Mel1TATA, AUR1-C MEL1 | ADE2, HIS3, MEL1, AUR1-C |
Y187 | ade2-101, trp1-901, leu2-3, 112, gal4Δ, gal80Δ, met–, URA3::GAL1UAS–Gal1TATA–LacZ, MEL1 | MEL1, LacZ |
Plasmid | Selectable markers (yeast, bacteria) | Additional information |
Plasmid pGBKT7 (bait vector) | TRP1, Kan | GAL4( 1–147)DNA-BD, TRP1, kanr, c-Myc epitope tag |
Plasmid pGADT7 (prey vector) | LEU2, Amp | GAL4(768–881)AD, LEU2, ampr, HA epitope tag |