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Table 2 Summary of affinity of bait-prey interactions

From: Identification of putative interactions between swine and human influenza A virus nucleoprotein and human host proteins

   

Growth on SD-His agar plates*

β-Gal activity**

Gene

Protein description

Prey clone

Human (30°C)

Human (37°C)

Swine (30°C)

Swine (37°C)

Human NP

Swine NP

PAICS

Phosphoribosylaminoimidazole carboxylase

R15

(++)

(+)

(++)

(++)

2.2

30.4

MSANTD3

Myb/SANT-like DNA-binding domain containing 3

R10

(++)

(++)

(++)

(++)

13.9

18.2

FLJ30306

PREDICTED: Homo sapiens uncharacterized LOC101059922, mRNA

R23

(+)

(+)

(++)

(++)

1.9

5.4

PA28B

Proteasome (prosome, macropain) activator subunit 2

R6

(++)

(−)

(++)

(+)

6.7

33.3

KCTD9

Potassium channel tetramerisation domain containing 9

R34

(+)

(+)

(++)

(++)

5.0

16.4

ACOT13

Acyl-CoA thioesterase 13

R11

(++)

(+)

(++)

(−)

6.9

15.5

TRA2B

Transformer 2 beta homolog (Drosophila)

R12

(++)

(++)

(++)

(++)

1.9

0.85

SLC30A9

Solute carrier family 30 (zinc transporter), member 9

R28

(++)

(+)

(++)

(+)

82.9

119.9

ATP1B1

ATPase, Na+/K+ transporting, beta 1 polypeptide

R7

(++)

(+)

(++)

(++)

3.4

4.3

CKAP5

Cytoskeleton associated protein 5

R29

(++)

(++)

(++)

(++)

70.1

90.3

  1. * Results from growth on SD-His protein interaction assay media are presented as: (++) normal growth, (+) reduced growth, and (-) no growth (Figure 2).
  2. ** Mean β-galalactosidase activity from at least eight replicates reported in Miller units (Figure 3).