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Figure 2 | Virology Journal

Figure 2

From: Identification of putative interactions between swine and human influenza A virus nucleoprotein and human host proteins

Figure 2

Maintenance of protein-protein interactions at 30°C and 37°C. Yeast cells containing both a bait plasmid and prey plasmid were spotted in duplicate onto agar plates and incubated at 30°C or 37°C. SD-Leu-Trp media serves as a growth control, and SD-His is protein interaction reporter media. Growth on SD-His is represented as normal growth (++), reduced growth (+), and no growth (−) (see Table 2). NC and PC represent negative and positive controls, respectively. Additional yeast strain negative controls include h- (human NP bait and empty prey vector) and s- (swine NP bait and empty prey vector).

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Virology Journal

ISSN: 1743-422X