Table 1 Primers set for amplification of the overlapping and truncated segments of VP1 gene
From: Identification of VP1 peptides diagnostic of encephalomyocarditis virus from swine
Fragment name | Primer sequence (5′-3′) | Fragment name | Primer sequence (5′-3′) |
|---|---|---|---|
F1:1 ~ 168aa | 5′-GATGGATCCGGAGTAGAAAACGC-3′ | F14:18 ~ 168aa | 5′-AATGGATCCGACTTTGTGGCTCAACC-3′ |
5′-TAACTCGAGCTAGCCTTCTGAGAG-3′ | 5′-ATTCTCGAGCTAGCCTTCTGAGAGGG-3′ | ||
F2:106 ~ 277aa | 5′-CGCGGATCCCTTAAATCCAAACAG-3′ | F15:17 ~ 168aa | 5′-TAAGGATCCGCTGACTTTGTGGC-3′ |
5′-GACCTCGAGTACTCTAGCATCAAG-3′ | 5′-ATTCTCGAGCTAGCCTTCTGAGAGGG-3′ | ||
F3:1 ~ 72aa | 5′-GATGGATCCGGAGTAGAAAACGC-3′ | F16:-100 ~ 23aa | 5′-TTAGGATCCGGAGCGGGCAAG-3′ |
5′-CACCTCGAGCTAGTCAGTATCACTG-3′ | 5′-ATCCTCGAGCTATGGTTGAGCCACAAA-3′ | ||
F4:38 ~ 105aa | 5′-TAAGGATCCGATAGGTCCAGTC-3′ | F17:-100 ~ 24aa | 5′-TTAGGATCCGGAGCGGGCAAG-3′ |
5′-GACCTCGAGCTACGGAAAGACTTTTG-3′ | 5′-CATCTCGAGCTAAACTGGTTGAGCCA-3′ | ||
F5:14 ~ 105aa | 5′-ATTGGATCCGACGCAACTGCTGA-3′ | F18:-100 ~ 25aa | 5′-TTAGGATCCGGAGCGGGCAAG-3′ |
5′-GACCTCGAGCTACGGAAAGACTTTTG-3′ | 5′-CATCTCGAGCTAGTAAACTGGTTGAGCC-3′ | ||
F6:7 ~ 105aa | 5′-CAGGGATCCAAAGGGGTCACTGAAAAC-3′ | F19:-100 ~ 26aa | 5′-TTAGGATCCGGAGCGGGCAAG-3′ |
5′-GACCTCGAGCTACGGAAAGACTTTTG-3′ | 5′-AGCCTCGAGCTACAAGTAAACTGGTTGA-3′ | ||
F7:4 ~ 168aa | 5′-GCAGGATCCAACGCTGAAAAAG-3′ | F20:43 ~ 168aa | 5′-TAAGGATCCATTGGTGCCTTCACCG-3′ |
5′-ATTCTCGAGCTAGCCTTCTGAGAGGG-3′ | 5′-ATTCTCGAGCTAGCCTTCTGAGAGGG-3′ | ||
F8:3 ~ 168aa | 5′-CCGGGATCCGAAAACGCTGAAAAA-3′ | F21:42 ~ 168aa | 5′-ATTGGATCCCCCATTGGTGCCTTC-3′ |
5′-ATTCTCGAGCTAGCCTTCTGAGAGGG-3′ | 5′-ATTCTCGAGCTAGCCTTCTGAGAGGG-3′ | ||
F9:2 ~ 168aa | 5′-CGAGGATCCGTAGAAAACGCTGAA-3′ | F22:41 ~ 168aa | 5′-ATAGGATCCAGTCCCATTGGTGCC-3′ |
5′-ATTCTCGAGCTAGCCTTCTGAGAGGG-3′ | 5′-ATTCTCGAGCTAGCCTTCTGAGAGGG-3′ | ||
F10:-100 ~ 6aa | 5′-TTAGGATCCGGAGCGGGCAAG-3′ | F23:-100 ~ 47aa | 5′-TTAGGATCCGGAGCGGGCAAG-3′ |
5′-GCACTCGAGCTATTCAGCGTTTTCTACT-3′ | 5′-TTCCTCGAGCTAGGTGAAGGCACCAAT-3′ | ||
F11:-100 ~ 7aa | 5′-TTAGGATCCGGAGCGGGCAAG-3′ | F24:-100 ~ 48aa | 5′-TTAGGATCCGGAGCGGGCAAG-3′ |
5′-GGCCTCGAGCTATTTTTCAGCGTTTTCTAC-3′ | 5′-ATACTCGAGCTACACGGTGAAGGCACC-3′ | ||
F12:20 ~ 168aa | 5′-TCAGGATCCGTGGCTCAACCAGTTTAC-3′ | F25:-100 ~ 49aa | 5′-TTAGGATCCGGAGCGGGCAAG-3′ |
5′-ATTCTCGAGCTAGCCTTCTGAGAGGG-3′ | 5′-ATACTCGAGCTACTTCACGGTGAAGGC-3′ | ||
F13:19 ~ 168aa | 5′-ACTGGATCCTTTGTGGCTCAACCAG-3′ | F26:-100 ~ 50aa | 5′-TTAGGATCCGGAGCGGGCAAG-3′ |
5′-ATTCTCGAGCTAGCCTTCTGAGAGGG-3′ | 5′-AACCTCGAGCTAGGACTTCACGGTGAA-3′ |