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Table 1 Primers set for amplification of the overlapping and truncated segments of VP1 gene

From: Identification of VP1 peptides diagnostic of encephalomyocarditis virus from swine

Fragment name Primer sequence (5′-3′) Fragment name Primer sequence (5′-3′)
F1:1 ~ 168aa 5′-GATGGATCCGGAGTAGAAAACGC-3′ F14:18 ~ 168aa 5′-AATGGATCCGACTTTGTGGCTCAACC-3′
5′-TAACTCGAGCTAGCCTTCTGAGAG-3′ 5′-ATTCTCGAGCTAGCCTTCTGAGAGGG-3′
F2:106 ~ 277aa 5′-CGCGGATCCCTTAAATCCAAACAG-3′ F15:17 ~ 168aa 5′-TAAGGATCCGCTGACTTTGTGGC-3′
5′-GACCTCGAGTACTCTAGCATCAAG-3′ 5′-ATTCTCGAGCTAGCCTTCTGAGAGGG-3′
F3:1 ~ 72aa 5′-GATGGATCCGGAGTAGAAAACGC-3′ F16:-100 ~ 23aa 5′-TTAGGATCCGGAGCGGGCAAG-3′
5′-CACCTCGAGCTAGTCAGTATCACTG-3′ 5′-ATCCTCGAGCTATGGTTGAGCCACAAA-3′
F4:38 ~ 105aa 5′-TAAGGATCCGATAGGTCCAGTC-3′ F17:-100 ~ 24aa 5′-TTAGGATCCGGAGCGGGCAAG-3′
5′-GACCTCGAGCTACGGAAAGACTTTTG-3′ 5′-CATCTCGAGCTAAACTGGTTGAGCCA-3′
F5:14 ~ 105aa 5′-ATTGGATCCGACGCAACTGCTGA-3′ F18:-100 ~ 25aa 5′-TTAGGATCCGGAGCGGGCAAG-3′
5′-GACCTCGAGCTACGGAAAGACTTTTG-3′ 5′-CATCTCGAGCTAGTAAACTGGTTGAGCC-3′
F6:7 ~ 105aa 5′-CAGGGATCCAAAGGGGTCACTGAAAAC-3′ F19:-100 ~ 26aa 5′-TTAGGATCCGGAGCGGGCAAG-3′
5′-GACCTCGAGCTACGGAAAGACTTTTG-3′ 5′-AGCCTCGAGCTACAAGTAAACTGGTTGA-3′
F7:4 ~ 168aa 5′-GCAGGATCCAACGCTGAAAAAG-3′ F20:43 ~ 168aa 5′-TAAGGATCCATTGGTGCCTTCACCG-3′
5′-ATTCTCGAGCTAGCCTTCTGAGAGGG-3′ 5′-ATTCTCGAGCTAGCCTTCTGAGAGGG-3′
F8:3 ~ 168aa 5′-CCGGGATCCGAAAACGCTGAAAAA-3′ F21:42 ~ 168aa 5′-ATTGGATCCCCCATTGGTGCCTTC-3′
5′-ATTCTCGAGCTAGCCTTCTGAGAGGG-3′ 5′-ATTCTCGAGCTAGCCTTCTGAGAGGG-3′
F9:2 ~ 168aa 5′-CGAGGATCCGTAGAAAACGCTGAA-3′ F22:41 ~ 168aa 5′-ATAGGATCCAGTCCCATTGGTGCC-3′
5′-ATTCTCGAGCTAGCCTTCTGAGAGGG-3′ 5′-ATTCTCGAGCTAGCCTTCTGAGAGGG-3′
F10:-100 ~ 6aa 5′-TTAGGATCCGGAGCGGGCAAG-3′ F23:-100 ~ 47aa 5′-TTAGGATCCGGAGCGGGCAAG-3′
5′-GCACTCGAGCTATTCAGCGTTTTCTACT-3′ 5′-TTCCTCGAGCTAGGTGAAGGCACCAAT-3′
F11:-100 ~ 7aa 5′-TTAGGATCCGGAGCGGGCAAG-3′ F24:-100 ~ 48aa 5′-TTAGGATCCGGAGCGGGCAAG-3′
5′-GGCCTCGAGCTATTTTTCAGCGTTTTCTAC-3′ 5′-ATACTCGAGCTACACGGTGAAGGCACC-3′
F12:20 ~ 168aa 5′-TCAGGATCCGTGGCTCAACCAGTTTAC-3′ F25:-100 ~ 49aa 5′-TTAGGATCCGGAGCGGGCAAG-3′
5′-ATTCTCGAGCTAGCCTTCTGAGAGGG-3′ 5′-ATACTCGAGCTACTTCACGGTGAAGGC-3′
F13:19 ~ 168aa 5′-ACTGGATCCTTTGTGGCTCAACCAG-3′ F26:-100 ~ 50aa 5′-TTAGGATCCGGAGCGGGCAAG-3′
5′-ATTCTCGAGCTAGCCTTCTGAGAGGG-3′ 5′-AACCTCGAGCTAGGACTTCACGGTGAA-3′