Inhibition of porcine reproductive and respiratory syndrome virus infection by recombinant adenovirus- and/or exosome-delivered the artificial microRNAs targeting sialoadhesin and CD163 receptors

Zhu, Li; Song, Hongqin; Zhang, Xinyu; Xia, Xiaoli; Sun, Huaichang

doi:10.1186/s12985-014-0225-9

Virology Journal

Table 1 The oligonucleotides used for construction of amiRNA expression vectors in this study

From: Inhibition of porcine reproductive and respiratory syndrome virus infection by recombinant adenovirus- and/or exosome-delivered the artificial microRNAs targeting sialoadhesin and CD163 receptors

Target	miRNA	Start	Double-stranded oligonucleotide sequence (5′→3′)
Sn	1	161	TGCTGAGTACCAGATGGCTGTGATGC GTTTTGGCCACTGACTGACGCATCACACATCTGGTACT
	1	161	CCTGAGTACCAGATGTGTGATGCGTCAGTCAGTGGCCAAAACGCATCACAGCCATCTGGTACTC
	2	367	TGCTGTTTGACATCTGACCAGCGGTT GTTTTGGCCACTGACTGACAACCGCTGCAGATGTCAAA
	2	367	CCTGTTTGACATCTGCAGCGGTTGTCAGTCAGTGGCCAAAACAACCGCTGGTCAGATGTCAAAC
	3	378	TGCTGACAACTGTGCCTTTGACATCT GTTTTGGCCACTGACTGACAGATGTCAGGCACAGTTGT
	3	378	CCTGACAACTGTGCCTGACATCTGTCAGTCAGTGGCCAAAACAGATGTCAAAGGCACAGTTGTC
	4	458	TGCTGAGTTGAAGTCCACCTCCATGC GTTTTGGCCACTGACTGACGCATGGAGGGACTTCAACT
	4	458	CCTGAGTTGAAGTCCCTCCATGCGTCAGTCAGTGGCCAAAACGCATGGAGGTGGACTTCAACTC
	5	461	TGCTGAGCAGTTGAAGTCCACCTCCA GTTTTGGCCACTGACTGACTGGAGGTGCTTCAACTGCT
	5	461	CCTGAGCAGTTGAAGCACCTCCAGTCAGTCAGTGGCCAAAACTGGAGGTGGACTTCAACTGCT
	6	669	TGCTGATCTCCTTCTGCATCCTGCGT GTTTTGGCCACTGACTGACACGCAGGACAGAAGGAGAT
	6	669	CCTGATCTCCTTCTGTCCTGCGTGTCAGTCAGTGGCCAAAACACGCAGGATGCAGAAGGAGATC
	7	1148	TGCTGAGAAGTAGAAGCCCGAATCCG GTTTTGGCCACTGACTGACCGGATTCGCTTCTACTTCT
	7	1148	CCTGAGAAGTAGAAGCGAATCCGGTCAGTCAGTGGCCAAAACCGGATTCGGGCTTCTACTTCTC
	8	1476	TGCTGTTCCCAAGAGAACTGCTGGCT GTTTTGGCCACTGACTGACAGCCAGCATCTCTTGGGAA
	8	1476	CCTGTTCCCAAGAGATGCTGGCTGTCAGTCAGTGGCCAAAACAGCCAGCAGTTCTCTTGGGAAC
	9	1510	TGCTGTGCATTGGCATGGAAGTCCAG GTTTTGGCCACTGACTGACCTGGACTTATGCCAATGCA
	9	1510	CCTGTGCATTGGCATAAGTCCAGGTCAGTCAGTGGCCAAAACCTGGACTTCCATGCCAATGCAC
CD163	1	94	TGCTGAGAACTAGTGACCAAGCAGGC GTTTTGGCCACTGACTGACGCCTGCTTTCACTAGTTCT
	1	94	CCTGAGAACTAGTGAAAGCAGGCGTCAGTCAGTGGCCAAAACGCCTGCTTGGTCACTAGTTCTC
	2	527	TGCTGTGAAGTTGTCATCACACACTG GTTTTGGCCACTGACTGACCAGTGTGTTGACAACTTCA
	2	527	CCTGTGAAGTTGTCAACACACTGGTCAGTCAGTGGCCAAAACCAGTGTGTGATGACAACTTCAC
	3	645	TGCTGCATACAAGATCATCAAACCA GGTTTTGGCCACTGACTGACCTGGTTTGGATCTTGTATG
	3	645	CCTGCATACAAGATCCAAACCAGGTCAGTCAGTGGCCAAAACCTGGTTTGATGATCTTGTATGC
	4	740	TGCTGTTAAGCAAATCACTCCAGCAT GTTTTGGCCACTGACTGACATGCTGGAGATTTGCTTAA
	4	740	CCTGTTAAGCAAATCTCCAGCATGTCAGTCAGTGGCCAAAACATGCTGGAGTGATTTGCTTAAC
	5	768	TGCTGACCACTCTCAGTTTCAGGTCT GTTTTGGCCACTGACTGACAGACCTGACTGAGAGTGGT
	5	768	CCTGACCACTCTCAGTCAGGTCTGTCAGTCAGTGGCCAAAACAGACCTGAAACTGAGAGTGGTC
	6	807	TGCTGTTCACTTCCAATCTTCCTGAA GTTTTGGCCACTGACTGACTTCAGGAATTGGAAGTGAA
	6	807	CCTGTTCACTTCCAATTCCTGAAGTCAGTCAGTGGCCAAAACTTCAGGAAGATTGGAAGTGAAC
	7	961	TGCTGAACACTGTCAAGCCAAATGTG GTTTTGGCCACTGACTGACCACATTTGTTGACAGTGTT
	7	961	CCTGAACACTGTCAACAAATGTGGTCAGTCAGTGGCCAAAACCACATTTGGCTTGACAGTGTTC
	8	998	TGCTGTACACTGCCAGAGAGCAGACT GTTTTGGCCACTGACTGACAGTCTGCTCTGGCAGTGTA
	8	998	CCTGTACACTGCCAGAGCAGACTGTCAGTCAGTGGCCAAAACAGTCTGCTCTCTGGCAGTGTAC
	9	1325	TGCTGAATTCTTGCAGTCCCAAAGAG GTTTTGGCCACTGACTGACCTCTTTGGCTGCAAGAATT
	9	1325	CCTGAATTCTTGCAGCCAAAGAGGTCAGTCAGTGGCCAAAACCTCTTTGGGACTGCAAGAATTC
	10	1486	TGCTGTAGTGACGAGACAAGCACAGC GTTTTGGCCACTGACTGACGCTGTGCTTCTCGTCACTA
	10	1486	CCTGTAGTGACGAGAAGCACAGCGTCAGTCAGTGGCCAAAACGCTGTGCTTGTCTCGTCACTAC
Control	amiRcon		TGCTGTAGTGACGAGACAAGCACAGC GTTTTGGCCACTGACTGACGCTGTGCTTCTCGTCACTA
Control	amiRcon		CCTGTAGTGACGAGAAGCACAGCGTCAGTCAGTGGCCAAAACGCTGTGCTTGTCTCGTCACTAC

The candidate amiRNAs were predicted by following the instructions for the web-based Block-iT^TM RNAi designer. The mature amiRNA and forward primer sequences for the detection of amiRNAs in rAd-transduced cells are indicated as italics.

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ISSN: 1743-422X

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