Figure 2

Difference in incorporation level in virions between Nef _mac239 -WT and Nef _mac239 -CP. pBRmac239Δnef/luc was cotransfected into HEK293 cells with either pNef_mac239-WT, pNef_mac239-CP, or an empty vector. For virion preparation, the supernatants were subjected to ultracentrifugation. Each viral pellet was then subjected to SDS-PAGE and western immunoblot analysis to detect Nef, Env, and p27, as described in Materials and Methods. The mock sample was electrophoresed on a lane distant from two lanes of the Nef_mac239-WT and Nef_mac239-CP.