Figure 6

Effect of hnRNP C1/C2 knockdown on DENV protein expression. Huh7 cells transfected with hnRNP C1/C2-specific siRNA (SP) or irrelevant negative control siRNA (IR) were infected with DENV at MOI 0.03. Cells were collected at 12, 24, 36 and 48 h post-infection and subjected to immunoblotting analysis using monoclonal antibodies specific against DENV NS1, DENV E and β-actin (internal control). Three independent experiments were performed. (A) Protein bands of DENV NS1, DENV E and β-actin in DENV-infected cells transfected with specific siRNA (SP) or control siRNA (IR). (B) The intensity of DENV NS1 and E bands in both IR and SP samples was first normalized to that of β-actin bands in the same set of samples. The obtained relative intensity of DENV NS1 and E in the SP samples was then normalized to that in the IR samples. Results show fold change of relative DENV NS1 and E protein expression in the SP samples compared with the corresponding IR samples. Asterisks indicate statistically significant differences (*p < 0.05) in DENV NS1 and E protein expression between the specific siRNA-transfected and control siRNA-transfected groups by unpaired t test.