Table 4 The primer sequence of the complete genome sequence
From: Characterization of one sheep border disease virus in China
Amplified fragments | primer | Primer sequences(5'- > 3') | Location (bp) | Fragment size |
|---|---|---|---|---|
F1 | BVDV-F | GCCATGCCCTTAGTAGGACTAGC | 1 ~ 23 | 2200 bp |
BDV-2300R | TATCAGGAAGGCTGTTGTCGA | 2255 ~ 2273 | ||
F2 | BDV-2240 F | TTGGTGGCCATACGAGACAAC | 2144 ~ 2164 | 1900 bp |
BDV-4140R | TGTCAAGATGAAGAATAGGGG | 4023 ~ 4043 | ||
F3 | BDV-4010 F | AAGCAGTGGCTACAATCCGTG | 3912 ~ 3932 | 1950 bp |
BDV-5960R | CATCTCTCCAATCCTCAGGTT | 5865 ~ 5885 | ||
F4 | BDV-5940 F | GCAGAAGCACCCTAGCATAGC | 5840 ~ 5860 | 2000 bp |
BDV-7940R | TATGACTACGCTCTCCAGCCG | 7929 ~ 7945 | ||
F5 | BDV-7885 F | GCCTTACGCATCTCAAGCCCTC | 7787 ~ 7808 | 2200 bp |
BDV-10110R | TGCCTCGTATGGGTGTATTTTC | 10001 ~ 10022 | ||
F6 | BDV-10010 F | CAGAGCATATGGTGTCAGCATATCAG | 9907 ~ 9932 | 2300 bp |
BDV-12326R | GGGGCTGTTAGGGTTTTTCCTTAATCC | 12201 ~ 12227 |