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Figure 2 | Virology Journal

Figure 2

From: The SARS coronavirus papain like protease can inhibit IRF3 at a post activation step that requires deubiquitination activity

Figure 2

SARS-CoV PLpro does not inhibit IRF3(5D) dimer formation. The ability of SARS-CoV PLpro to inhibit IRF3(5D) dimer formation was assayed using native PAGE gels. A. HEK293T cells were transfected with either Flag-tagged IRF3, HA-tagged PLpro or Flag-tagged IRF3(5D) in combination or alone. For Flag-tagged IRF3 transfections, SeV was added at 18 hours post transfection for 6 hours before cells were lysed. 5ul of total lysate was run on a SDS/PAGE gel to assay input lysate levels. B. Lysate from transfections in A were ran on Native PAGE gels for IRF3 dimer visualization in the noted combinations of transfected plasmids, including a Flag-tagged IRF3 + SeV infected sample that was boiled before loading to show monomer size. C. Lysate from transfections in A were ran on Native PAGE gels for IRF3(5D) dimer visualization in the noted combinations of transfected plasmids, including a Flag-tagged IRF3(5D) that was boiled before loading to show monomer size. d = dimer, m = monomer.

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Virology Journal

ISSN: 1743-422X