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Figure 4 | Virology Journal

Figure 4

From: Detailed morphological characterisation of Hendra virus infection of different cell types using super-resolution and conventional imaging

Figure 4

Porcine aortic endothelial cells (PAEC) and bat-derived cells (PaLu) infected with HeV and fixed at 18 hpi for confocal microscopy. a) PAEC cells labelled with anti-HeV G antibody mAb m102.4 detected with Alexa 568-conjugated anti-human antibody (red) showing an ER-like distribution of the HeV G protein and small dots. b) PAEC cells labelled with mouse anti-HeV M protein detected with anti-mouse antibody conjugated to Alexa 488 (green) c) overlaid image of a) and b) showing colocalisation of the M and G in the dots (yellow). d) PaLu cells labelled as for PAEC cells. Image is merged M (green) and G (red). Nuclei were labelled with DAPI (blue). Fluorescence images were merged with DIC images. Scale bars: a, b, c) =20 μm d) =20 μm.

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Virology Journal

ISSN: 1743-422X