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Figure 7 | Virology Journal

Figure 7

From: Imaging analysis of human metapneumovirus-infected cells provides evidence for the involvement of F-actin and the raft-lipid microdomains in virus morphogenesis

Figure 7

Biochemical evidence for an interaction between the F and G proteins on co-transfected cells. (A) Cells were either transfected with either (1) pCAGGS/G-FLAG or (2) pCAGGS/G-FLAG and pCAGGS/F-cmyc, and then surface-biotinylated and immunoprecipitated (IP) with anti-FLAG or (B) cells were either transfected with either (1) pCAGGS/F-cmyc or (2) pCAGGS/G-FLAG and pCAGGS/F-cmyc, and then surface biotinylated and immunoprecipitated (IP) with anti-cmyc. (C) Cells were transfected with pCAGGS, and then surface-biotinylated, and immunoprecipitated (IP) with either anti-FLAG or anti-cymc. Biotinylated proteins species corresponding to the G and F proteins are indicated. (D) (i) pCAGGS/G-FLAG and pCAGGS/F-cmyc, co-transfected cells were immunoprecipitated (IP) with anti-FLAG and immunoblotted (IB) with anti-cmyc or (ii) cells were immunoprecipitated (IP) with anti-cmyc and immunoblotted with anti-FLAG. Proteins species corresponding to the G protein (G and G*) and F protein (F) are indicated.

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Virology Journal

ISSN: 1743-422X