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Figure 1 | Virology Journal

Figure 1

From: Infection of Porcine Circovirus 2 (PCV2) in Intestinal Porcine Epithelial Cell Line (IPEC-J2) and Interaction between PCV2 and IPEC-J2 Microfilaments

Figure 1

Infection of PCV2 in IPEC-J2 Cells. (A, B, C and D). Electron microscopic analysis of ultrathin sections of IPEC-J2 cells either without PCV2 (A , control ), or infected with PCV2 at 3×102.5 TCID50/ml for 1 h (B) and 48 h (C and D). Black arrows indicate virus. (E) Flow cytometry analysis of mean fluorescence intensity of PCV2 in IPEC-J2 cells either without PCV2 (- , control ), or infected with PCV2 at 3×102.5 TCID50/ml for 1, 24, 48 and 72 h. PCV2 was detected using a PCV2 capsid protein rabbit polyclonal antibody and a DyLight488 goat anti-rabbit IgG secondary antibody. Values are means×SEM for three separate experiments. (F) Viral growth curve. IPEC-J2 cells were infected with PCV2 at 3×102.5 TCID50/ml. Supernatants and cells were harvested at 6, 12, 24, 48, 72, 96 and 120 h p.i. To obtain cell-associated infectivity, viral DNAs were quantitated by qRT-PCR assay. Values are means×SEM for three separate experiments.

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Virology Journal

ISSN: 1743-422X