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Figure 1 | Virology Journal

Figure 1

From: The new temperature-sensitive mutation PA-F35S for developing recombinant avian live attenuated H5N1 influenza vaccine

Figure 1

Temperature-sensitive phenotype of the recombinant viruses. Six 10-day-old embryonated SPF chicken eggs were inoculated with 103 EID50 (0.2 ml/egg) of the recombinant viruses and incubated at 37°C and 41°C for 48 hours. Viral titers were determined by log10EID50/ml at 37°C. The detection limit is 0.7 log10EID50/ml. The statistical analysis was performed using Student’s t test (, p < 0.01; , p < 0.05).

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