Table 1 Target genes and primer sequences for respiratory pathogens
From: Respiratory viruses in children hospitalized for acute lower respiratory tract infection in Ghana
| Virus | Target gene | Function | Oligonucleotide sequence |
|---|---|---|---|
| RSVa (A/B) | Matrix gene [17] | Forward primer | 5'-GGAAACATACGTGAACAAGCTTCA |
| Reverse primer A | 5'-CATCGTCTTTTTCTAAGACATTGTATTGA | ||
| Reverse primer B | 5'-TCATCATCTTTTTCTAGAACATTGTACTGA | ||
| Probe | 6FAM-TGTGTATGTGGAGCCTT- MGBNFQ | ||
| Adenovirus | Hexon gene [18] | Forward primer | 5'-GCCACGGTGGGGTTTCTAAACTT |
| Reverse primer | 5'-GCCCCAGTGGTCTTACATGCACAT | ||
| Probe | 6FAM-TGCACCAGACCCGGGCTCAGGTACTCCGA-TAMRA | ||
| PIV Ib | Polymerase gene [19] | Forward primer | 5'-ACAGATGAAATTTTCAAGTGCTACTTTAGT |
| Reverse primer | 5'-GCCTCTTTTAATGCCATATTATCATTAGA | ||
| Probe | 6FAM-ATGGTAATAAATCGACTCGCT- MGBNFQ | ||
| PIV IIc | Polymerase gene [19] | Forward primer | 5'-TGCATGTTTTATAACTACTGATCTTGCTAA |
| Reverse primer | 5'-GTTCGAGCAAAATGGATTATGGT | ||
| Probe | 6FAM-ACTGTCTTCAATGGAGATAT- MGBNFQ | ||
| PIV IIId | Matrix gene [19] | Forward primer | 5'-TGCTGTTCGATGCCAACAA |
| Reverse primer | 5'-ATTTTATGCTCCTATCTAGTGGAAGACA | ||
| Probe | 6FAM-TTGCTCTTGCTCCTCA- MGBNFQ | ||
| Influenza A | Matrix gene [20] | Forward primer A1 | 5'-GGACTGCAGCGTAGACGCTT |
| Forward primer A2 | 5'-CATCCTGTTGTATATGAGGCCCAT | ||
| Reverse primer | 5'-CATTCTGTTGTATATGAGGCCCAT | ||
| Probe | 6FAM-CTCAGTTATTCTGCTGGTGCACTTGCCA- MGBNFQ | ||
| Influenza B | Hemagglutinin (HA)gene [20] | Forward primer | 5'-AAATACGGTGGATTAAATAAAAGCAA |
| Reverse primer | 5'-CCAGCAATAGCTCCGAAGAAA | ||
| Probe | 6FAM-CACCCATATTGGGCAATTTCCTATGGC- MGBNFQ |