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Table 1 Target genes and primer sequences for respiratory pathogens

From: Respiratory viruses in children hospitalized for acute lower respiratory tract infection in Ghana

Virus Target gene Function Oligonucleotide sequence
RSVa (A/B) Matrix gene [17] Forward primer 5'-GGAAACATACGTGAACAAGCTTCA
   Reverse primer A 5'-CATCGTCTTTTTCTAAGACATTGTATTGA
   Reverse primer B 5'-TCATCATCTTTTTCTAGAACATTGTACTGA
   Probe 6FAM-TGTGTATGTGGAGCCTT- MGBNFQ
Adenovirus Hexon gene [18] Forward primer 5'-GCCACGGTGGGGTTTCTAAACTT
   Reverse primer 5'-GCCCCAGTGGTCTTACATGCACAT
   Probe 6FAM-TGCACCAGACCCGGGCTCAGGTACTCCGA-TAMRA
PIV Ib Polymerase gene [19] Forward primer 5'-ACAGATGAAATTTTCAAGTGCTACTTTAGT
   Reverse primer 5'-GCCTCTTTTAATGCCATATTATCATTAGA
   Probe 6FAM-ATGGTAATAAATCGACTCGCT- MGBNFQ
PIV IIc Polymerase gene [19] Forward primer 5'-TGCATGTTTTATAACTACTGATCTTGCTAA
   Reverse primer 5'-GTTCGAGCAAAATGGATTATGGT
   Probe 6FAM-ACTGTCTTCAATGGAGATAT- MGBNFQ
PIV IIId Matrix gene [19] Forward primer 5'-TGCTGTTCGATGCCAACAA
   Reverse primer 5'-ATTTTATGCTCCTATCTAGTGGAAGACA
   Probe 6FAM-TTGCTCTTGCTCCTCA- MGBNFQ
Influenza A Matrix gene [20] Forward primer A1 5'-GGACTGCAGCGTAGACGCTT
   Forward primer A2 5'-CATCCTGTTGTATATGAGGCCCAT
   Reverse primer 5'-CATTCTGTTGTATATGAGGCCCAT
   Probe 6FAM-CTCAGTTATTCTGCTGGTGCACTTGCCA- MGBNFQ
Influenza B Hemagglutinin (HA)gene [20] Forward primer 5'-AAATACGGTGGATTAAATAAAAGCAA
   Reverse primer 5'-CCAGCAATAGCTCCGAAGAAA
   Probe 6FAM-CACCCATATTGGGCAATTTCCTATGGC- MGBNFQ
  1. RSVa, Respiratory syncytial virus; PIV Ib, Parainfluenza virus type 1; PIV IIc, Parainfluenza virus type 2, PIV IIId, Parainfluenza virus type 3.