Figure 6
From: Immune response to a potyvirus with exposed amino groups available for chemical conjugation
IFNγ and IL4 levels in stimulated splenocytes from mice immunized with TEV. IFNγ and IL4 levels in the supernatants of cultured splenocytes from immunized or nonimmunized mice following restimulation with TEV or no treatment were measured by ELISA. The graphs show the IFNγ (A) and IL4 concentrations (B) in picograms per milliliter (pg/mL) for each treatment: nonimmunized/unstimulated (C-), nonimmunized/stimulated (C+), immunized/unstimulated (TEV-), and immunized/stimulated (TEV+). The cytokine levels were calculated using standard curves generated with known concentrations of recombinant proteins. The data are presented as the mean cytokine concentrations ± SD of triplicate measurements. "Undetectable" (§) means below the lowest point on the standard curve.