Figure 5

In vitro proliferation assay of T-cell subpopulations derived from spleen cells of TEV-vaccinated mice. Mononuclear cells were isolated from the spleens of TEV-immunized (TEV) and nonimmunized (C) mice. To evaluate lymphocyte proliferation, the cultured cells of both groups were either unstimulated (-) or stimulated with 5 μg of virus (+). The collected cells from each treatment were triple-stained with fluorescent antibodies (PE-Cy5-labeled anti-CD3, FITC-labeled anti-CD4, and PE-labeled anti-CD8) and analyzed in a flow cytometer. The graphs show the relative percentages of double-stained cells, CD3⁺/CD4⁺ for helper T cells (A) and CD3⁺/CD8⁺ for cytotoxic T cells (B). The results are expressed as the means ± standard deviations (SD) of the corresponding measurements. Statistically significant differences are indicated with an asterisk (*P < 0.05).