Figure 4
From: Immune response to a potyvirus with exposed amino groups available for chemical conjugation
Analysis of the antibody responses with ELISA. Serum samples were collected from each group (C and TEV) at different times (see the immunization and bleeding schedule in Figure 3), diluted 1:40 with PBS, and tested for anti-TEV IgG1 (A), IgG2a (B), and IgG2b (C) antibody isotypes. Aliquots (5 μg per well) of TEV were fixed to the plate. Readings were performed with the TMB colorimetric substrate at 370 nm until reaction saturation. The results are expressed as the means ± standard deviations (SD) of the corresponding measurements. Statistically significant differences are indicated with an asterisk (*P < 0.05).