Figure 3
From: Immune response to a potyvirus with exposed amino groups available for chemical conjugation
Immunization and serum collection schedule. To evaluate the TEV-associated immune response in a mouse model, two groups of five female Balb/c mice were tested, designated C and TEV. Pure TEV (25 μg) or an equal volume of 20 mM Tris (pH 8.0) were administered to the TEV or C group, respectively. The mice were immunized intraperitoneally (IP) on day 0 and boosted on day 14. During the experiment, the mice were bled three times, on days -7 (B1), 13 (B2), and 27 (B3). The animals were killed at the end of the experiment on day 28, before splenectomy.