Figure 3

INA-treatment results in an increase in pelletablehemagglutinin recognized by neutralizing antibodies. Influenza virus (PR8) was treated as indicated below the figure. "Controls" refers to virus after the treatment specified (not immunoprecipitated). "Immunoprecipitated samples", as indicated, were treated as specified and then pelleted through sucrose. All pellets were then resuspended in 1% Triton and immunoprecipitated using an anti-HA antibody. Mock-IP was also performed without the addition of antibody to assess background binding to the beads. Western blot was probed using anti-HA2. Image shown is representative of duplicate experiments.