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Table 2 Bacterial strains, phages and plasmids used in this study

From: Characterization and determination of holin protein of Streptococcus suis bacteriophage SMP in heterologous host

Strains, phages or plasmids Genotype and relevant features Source
Strains DH5α E. coli F- endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80dlac ZΔM15 Δ(lacZYA- argF)U169, hsdR17(rK- mK+), λ- Tiangen
BL21(DE3) E. coli F - ompT hsdS(r B - m B - ) gal dcm (DE3) Tiangen
BL21(DE3) plysS E. coli F-ompT hsdS(r B -m B -) gal dcm (DE3) pLysS (CmR) Tiangen
VCS 257 DP50 sup F[supE44 supF58 hsd53(rB mB)dap D8lacY1 glnV44 Δ(gal-uvrB)47 tyrT58 gyrA29 tonA53 Δ(thyA57)] Stratagene
SS2-H Phages Streptococcus suis, serotype 2 Lab stock
SMP Wild type phage of S. suis Lab stock
λ c I857 Sam 7 cIts857, Sam7,Lac promoter for expression of cloned genes, phage of E. coli Stratagene
Plasmids   
pET-32a(+) Expression vector containing hybrid T7-lacPO promoter, Φ10 ribosome binding site, and lacI gene (pBR322 derivative), ampicillin resistance Novagen
pACYC184 Low copynumber cloning vector; p15A ori; Tc+; Cm+ Lab stock
pEXH1 Derivative of pET-32a(+); whole ORF of HolSMP gene, HolSMP(429), inserted behind RBS of pET-32a(+) This study
pEXL Derivative of pET-32a(+); LySMP fragment inserted behind RBS of pET-32a(+) This study
pEX Derivative of pET-32a(+); pEXH1 (Δ HolSMP) This study
pACEXL Hin d III-Sph I fragment of pEXL subcloned into Hin d III-Sph I sites of pACYC184 This study
pACEX Hin d III-Sph I fragment of pEX subcloned into Hin d III-Sph I sites of pACYC184 This study