Table 2 Bacterial strains, phages and plasmids used in this study
Strains, phages or plasmids | Genotype and relevant features | Source |
|---|---|---|
Strains DH5α | E. coli F- endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80dlac ZΔM15 Δ(lacZYA- argF)U169, hsdR17(rK- mK+), λ- | Tiangen |
BL21(DE3) | E. coli F - ompT hsdS(r B - m B - ) gal dcm (DE3) | Tiangen |
BL21(DE3) plysS | E. coli F-ompT hsdS(r B -m B -) gal dcm (DE3) pLysS (CmR) | Tiangen |
VCS 257 | DP50 sup F[supE44 supF58 hsd53(rB mB)dap D8lacY1 glnV44 Δ(gal-uvrB)47 tyrT58 gyrA29 tonA53 Δ(thyA57)] | Stratagene |
SS2-H Phages | Streptococcus suis, serotype 2 | Lab stock |
SMP | Wild type phage of S. suis | Lab stock |
λ c I857 Sam 7 | cIts857, Sam7,Lac promoter for expression of cloned genes, phage of E. coli | Stratagene |
Plasmids | ||
pET-32a(+) | Expression vector containing hybrid T7-lacPO promoter, Φ10 ribosome binding site, and lacI gene (pBR322 derivative), ampicillin resistance | Novagen |
pACYC184 | Low copynumber cloning vector; p15A ori; Tc+; Cm+ | Lab stock |
pEXH1 | Derivative of pET-32a(+); whole ORF of HolSMP gene, HolSMP(429), inserted behind RBS of pET-32a(+) | This study |
pEXL | Derivative of pET-32a(+); LySMP fragment inserted behind RBS of pET-32a(+) | This study |
pEX | Derivative of pET-32a(+); pEXH1 (Δ HolSMP) | This study |
pACEXL | Hin d III-Sph I fragment of pEXL subcloned into Hin d III-Sph I sites of pACYC184 | This study |
pACEX | Hin d III-Sph I fragment of pEX subcloned into Hin d III-Sph I sites of pACYC184 | This study |