Table 2 Depicts the primers designed and employed to amplify gC region
GENE SUB REGION | PRIMER ORIENTATION | PRIMER SEQUENCE (5'-3') |
|---|---|---|
gC1 | Forward | CGTGTGGAGGTCGTTTTTCAGT |
Reverse | GTGGTGTTGTTCTTGGGTTTGG | |
gC2 | Forward | AAACCCCAACAATGTCACACAAAAC |
Reverse | CCAAGTAATACATTCCCTGGGTCG | |
gC3 | Forward | GACCCAGGGAATGTATTACT |
Reverse | GTCCTCGAACCAGACAAACT | |
gC4 | Forward | AGTTTGTCTGGTTCGAGGAC |
Reverse | GTCATCGGCAGGTGAAGGTC | |
gC5 | Forward | ACCATCACCATGGAATTTGG |
Reverse | ATGACCTGAGGGGAGAGAGG |