Figure 6
From: Characterization and specificity of the linear epitope of the enterovirus 71 VP2 protein
Antigen capture ELISA. 500 ng of rabbit polyclonal anti-EV71 antibody was coated in each well of the ELISA plate. EV71-B5 virus antigen was added at dilutions of 10^7, 10^6, and 10^5 TCID50 units per well and detected by 1 ug of MAb 7C7 followed by HRP labeled anti-mouse antibody. DMEM media instead of 7C7 was used as a negative control. O-phenylenediamine as a substrate was used for color development and the OD was measured at 490 nm. The anti-VP2 MAb 7C7 could detect as little as 10^5 TCID50 units of EV71 captured by polyclonal serum in this ELISA assay.