Figure 1
From: Characterization and specificity of the linear epitope of the enterovirus 71 VP2 protein
Characterization of MAb 7C7. IFA (A), Western blot (B), Dot blot (C) of EV71 virus with MAb 7C7. (A) IFA of EV71 infected Vero cells. Cells were labeled with MAb 7C7 followed by anti-mouse FITC secondary antibody. Top panel: Vero cells infected with EV71-B5 (NUH0083). Bottom panel: Non-infected Vero cells. (B) Western blot of sucrose purified EV71-C4 strain (75-Yamagata-03). Blots were labeled with primary antibodies and HRP coupled secondary antibodies and bands were developed by ECL. Lane 1: Polyclonal guinea pig anti-EV71 (B5 strain) antibody detects all three capsid proteins VP0/2 (arrows), VP1 and VP3 (arrowheads). Lane 2: Monoclonal anti-VP1 antibody (in press) specifically labels the VP1 band (arrowhead). Lane 3: MAb 7C7 recognizes VP2 at about 28 kDa and its precursor VP0 at around 35 kDa (arrows). (C) Sensitivity of MAb 7C7 in a dot blot assay. EV71-B5 virus was dotted at 10-fold dilutions of TCID50 units per dot, labeled by MAb 7C7 and detected by HRP-coupled secondary antibody. H7N1 virus served as negative control. The detection limit was around 10^5 TCID50 units.