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Table 1 Sequences of primers used in cDNA synthesis, PCR and sequencing in this study

From: Genetic relationships and epidemiological links between wild type 1 poliovirus isolates in Pakistan and Afghanistan

Primer

Location (nt)

Sequence

Y7

2396-2418

5'-GGGTTTGTGTCAGCCTGTAATGA-3'

Q8

3504-3485

5'-AAGAGGTCTCTRTTCCACAT-3

PV1A *

2954-2935

5'-TTIAIIGCRTGICCRTTRTT-3'

PV4 S *

2830-2849

5'-ACITAYAARGAYACIGTICA-3'

  1. * Inosine Containing Primeers, Use degenerate PCR Condition